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Cell Insert

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Membrane:
  


packing specification Inserts/plate Insert Diameter(mm) Well Volume(ml) Insert Volume(ml) Insert Membrane Growth Area(cm²)
6 6 inserts/6well 24 2.6 1.5 4.67
12 12 inserts/6well 12 1.5 0.5 1.12
24 12 inserts/6well 6.5 0.6 0.1 0.33
100mm dish 1 inserts/6well 75 13 9 44

Pore Size(um) Membrane Density(well/cm²
0.4 1x108
3.0 2x106
5.0 4x105
8.0 1x105

50% Transparent PC Membrane Cell Insert, Tissue Culture Treated(Individually packaged in peelable Tyvek film and Blister,10/case)
Cat.No. Type(Well) Pore Size(um)
723001 6 3.0
724001 12 3.0
725001 24 3.0
723101 6 0.4
724101 12 0.4
725101 24 0.4
724201 12 5.0
725201 24 5.0
723301 6 8.0
724301 12 8.0
725301 24 8.0
726001 100mm dish 3.0

50% Transparent PC Membrane Cell Insert, Non-treated(Individually packaged in peelable Tyvek film and Blister,10/case)
Cat.No. Type(Well) Pore Size(um)
723011 6 3.0
724011 12 3.0
725011 24 3.0
723111 6 0.4
724111 12 0.4
725111 24 0.4
724211 12 5.0
725211 24 5.0
723311 6 8.0
724311 12 8.0
725311 24 8.0

Transparent PET Membrane Cell Insert, Tissue Culture Treated(Individually packaged in peelable Tyvek film and Blister,10/case)
Cat.No. Type(Well) Pore Size(um)
723121 6 0.4
724121 12 0.4
725121 24 0.4
723021 6 3.0
724021 12 3.0
725021 24 3.0

Transparent PET Membrane Cell Insert, Non-treated(Individually packaged in peelable Tyvek film and Blister,10/case)
Cat.No. Type(Well) Pore Size(um)
723131 6 0.4
724131 12 0.4
725131 24 0.4
723031 6 3.0
724031 12 3.0
725031 24 3.0

Multiple well plate  or dish is made from PS

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines. 
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.


·Innovative hanging design facilitates pipetting.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
·Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy. 
· Transparent PET membrane is easy to observe.
·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.

Attention:
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines for us:
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.