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Cell Culture Inserts

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Membrane:
  



packing specification Inserts/plate Insert Diameter(mm)
Volume of Each Well (mL)
Inner Volume of Insert (mL)
Membrane Growth Area of Insert (cm²)
6 6 inserts/6well 24 2.6 1.5 4.67
12 12 inserts/6well 12 1.5 0.5 1.12
24 12 inserts/6well 6.5 0.6 0.1 0.33
100mm dish 1 inserts/6well 75 13 9 44


Pore Size of Membrane (μm)
Pore Density(well/cm²
0.4 1x108
1.0
2x107
3.0 2x106
5.0 4x105
8.0 1x105

Cell Culture Inserts, PC Membrane

Individually packaged in peelable Tyvek film and Blister, Sterile

TC Treated Cat. No.

Non-Treated   Cat. No.

Product   Description

Pore   Size

(μm)

Membrane Transparence

/Pack

/Case

723001

723011

6 Cell Culture Inserts+6 Well Plate

3.0

Translucent

6

60

724001

724011

12 Cell Culture Inserts+12 Well Plate

3.0

Translucent

12

120

725001

725011

12 Cell Culture Inserts+24 Well Plate

3.0

Translucent

12

120

723101

723111

6 Cell Culture Inserts+6 Well Plate

0.4

Opaque

6

60

724101

724111

12 Cell Culture Inserts+12 Well Plate

0.4

Opaque

12

120

725101

725111

12 Cell Culture Inserts+24 Well Plate

0.4

Opaque

12

120

724201

724211

12 Cell Culture Inserts+12 Well Plate

5.0

Translucent

12

120

725201

725211

12 Cell Culture Inserts+24 Well Plate

5.0

Translucent

12

120

723301

723311

6 Cell Culture Inserts+6 Well Plate

8.0

Translucent

6

60

724301

724311

12 Cell Culture Inserts+12 Well Plate

8.0

Translucent

12

120

725301

725311

12 Cell Culture Inserts+24 Well Plate

8.0

Translucent

12

120

726001

/

100mm Cell Culture Insert-Dish

3.0

Translucent

1

10

 

 Cell Culture Inserts, PET Membrane

Individually packaged in peelable Tyvek film and Blister, Sterile

TC Treated Cat. No.

Non-Treated   Cat. No.

Product   Description

Pore   Size

(μm)

Membrane Transparence

/Pack

/Case

723121

723131

6 Cell Culture Inserts+6 Well Plate

0.4

Opaque

6

60

724121

724131

12 Cell Culture Inserts+12 Well Plate

0.4

Opaque

12

120

725121

725131

12 Cell Culture Inserts+24 Well Plate

0.4

Opaque

12

120

723021

723031

6 Cell Culture Inserts+6 Well Plate

3.0

Translucent

6

60

724021

724031

12 Cell Culture Inserts+12 Well Plate

3.0

Translucent

12

120

725021

725031

12 Cell Culture Inserts+24 Well Plate

3.0

Translucent

12

120

723421

723431

6 Cell Culture Inserts+6 Well Plate

1.0

Transparent

6

60

724421

724431

12 Cell Culture Inserts+12 Well Plate

1.0

Transparent

12

120

725421

725431

12 Cell Culture Inserts+24 Well Plate

1.0

Transparent

12

120

723321

723331

6 Cell Culture Inserts+6 Well Plate

8.0

Transparent

6

60

724321

724331

12 Cell Culture Inserts+12 Well Plate

8.0

Transparent

12

120

725321

725331

12 Cell Culture Inserts+24 Well Plate

8.0

Transparent

12

120

 




Multiple well plate  or dish is made from PS


Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines. 
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.



·Innovative hanging design facilitates pipetting.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.


Attention:
• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
•During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
•Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.


Guidelines for us:
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight. • Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.