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Bacteria Total RNA Purification Kit (Spin Column)

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Bacteria Total RNA Purification Kit (Spin Column)

[Product Code ]

208141

[Specification]

50 reactions

[Introduction]

The kit can be used to extract total RNA from cultured Gram-negative and Gram-positive bacteria. With the purification technology of silica membrane, the kit can complete extraction in 30-40 mins, avoid the application of phenol, chloroform and toxic agents, and eliminate DNA, impure protein and other impurities to the maximum extent. The kit is applicable to multiple downstream experiments such as RT-qPCR, chip analysis, Northern Blot, Dot Blot, PolyA filtration, in vitro translation, RNase protection analysis and molecular clone.

[Contents]

Product CompositionContentQuantity
Buffer TE15 mL / bottle1 bottle
Proteinase K1.04 mL / tube1 tube
Buffer BARB20 mL / bottle1 bottle
Buffer BARW36 mL / bottle1 bottle
Buffer CFW212 mL / bottle1 bottle
RNase-Free Water7 mL / bottle1 bottle
Spin Column NP2050 pieces / bag1 bag
Collection tube50 pieces / bag1 bag
Instructions1 copy1 copy

[Shipping & Storage Conditions and Shelf Life]

Transported at room temperature and stored at room temperature away from light. The shelf life is 12 months.

Production Date: Refer to the label.

Expiration Date: Refer to the label.

[Equipment and Reagents Prepared by User]

1. Lysozyme

2. DNase I (optional)

3.  Absolute ethyl alcohol

4. Centrifugal tube of 1.5 mL

5.  Vortex mixer

6.  Centrifugal machine: maximum speed of revolution 12,000 rpm (~13,400×g)

[Reagent Preparation]

1. Dissolve lysozyme by Buffer TE until its concentration reaches 15 mg/ mL and store the dissolved lysozyme at temperature of -20 °C. Please prepare lysozyme yourself for it is not available in the kit. (Lysozyme of Sangon Biotech, Order No.: A610308; Sigma, Cat. No. L7651 is recommended).

Attention: Lysozyme should not be frozen or thawed repeatedly. It is suggested to pack the dissolved lysozyme separately before storage or use it promptly upon preparation. Other enzymes may be more effective and applicable to some bacteria. (Lysostaphin, for example, is more effective and applicable to rupture cytoderm of staphylococcus aureus.)