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RT-Bst LAMP MasterMix (with SYTO-9)

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RT-Bst LAMP MasterMix (with SYTO-9)

[Product Code]

210701


[Specification]

50 reactions


[Introduction]

Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique widely used in the detection of target nucleic acid. The kit provides a simple solution for LAMP and RT-LAMP assays for DNA and RNA detection. LAMP and RT-LAMP are common isothermal amplification techniques, which use LAMP-specific primers (provided by user) and strand displacement polymerase to rapidly detect target nucleic acid. The kit contains 2.5×LAMP Mix and RT-LAMP enzyme Mix, in which the 2.5×LAMP Mix contains reaction buffer, fluorescent dye, Mg2+, dNTPs, etc., and is used together with fluorescence quantitative PCR instrument or thermostatic fluorescence detector. When used, only primers and templates need to be added for nucleic acid isothermal amplification.


[Contents]

Product composition50 Reactions
2.5×LAMP Mix500 μL
RT-LAMP enzyme Mix50 μL
Instruction For Use1 pcs


[Shipping and Storage Conditions]

The kit needs to be transported frozen and the components of the kit should be stored

away from light under -18°C.

Period of validity: 12 months.

Manufacturing date: see label.

Expiration Date: see label.


[Protocol]

1. The components were dissolved at room temperature and centrifuged briefly at 6000 RPM for 30 s. After mixing, they were set aside at 2-8°C.

2. Prepare LAMP reaction mixture in centrifuge tube as shown below (the whole process should be carried out in ice box): 

ComponentVolume (μL)
2.5×LAMP Mix10

RT-LAMP enzyme Mix

1
10xLAMP Primer Mix 2.5

Nucleasee free ddH2O

Add water to a volume of 20 μL