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Nucleic Acid Extraction Kit

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 Product Application
The kit is composed of reagents for nucleic acid extraction (magnetic beads method), suitable for extracting genomic nucleic acid DNA and RNA of bacteria, viruses and parasites from samples. It is designed for 96-well plate automatic platforms to help dramatically improve the throughput of sample extraction.

 Principle of Extraction
The kit uses a unique magnetic bead and buffer system. The surface of the magnetic bead is modified with special chemical groups, which have extremely strong enrichment ability for DNA/RNA under certain conditions and can reversibly release DNA/RNA when the conditions are changed to rapidly separate and purify DNA/RNA and to remove impurities like proteins as far as possible. The extracted high-quality nucleic acids can be used for serial experiments like PCR, RT-PCR, gene sequencing, etc.

 Applicable Models
King Fisher Presto


King Fisher Flex


 List of Product Composition

Product name

Amount

Capacity

Components of Pre-filled Solution

96-tip Plastic Comb

1 pcs

/

/

Sample Plate

1 pcs

400μL×96 wells

Guanidine salt, surfactant, EDTA, buffer, etc.

Magnetic Bead Plate

1 pcs

200μL×96 wells

Magnetic beads, buffer, etc.

Washing Plate 1

1 pcs

600μL×96 wells

Guanidine salt, EDTA, etc.

Washing Plate 2

2 pcs

600μL×96 wells

75% ethanol

Eluting Plate

1 pcs

100μL×96 wells

TE buffer

Protein K

1 mL x 2 pcs

/

Protein K (PK)


 Product Advantages

1.  Transport at Ambient Temperature：The kit contains the proprietary “proteinase K” that can be stored at ambient temperature, therefore the whole kit can be transported and stored at ambient temperature.
2.  Easy to Use: sub-packed reagents, use directly after tearing the sealing film.
3.  Good Sealing: high-stickiness sealing film, to further prevent fluid leakage during transport.
4.  High Extraction Purity of Sample Nucleic Acid, Low Inter-lot Difference

 List of Product Composition


 Performance Parameter

1.   Extraction Purity: A260/280= 1.7-2.0, A260/230 >1.9

2.   Yield of Nucleic Acid: >85%

3.   Extraction Time: around 45 min

4.   Extracted Number: 96 samples for each run

5.   Sample Adding Amount: 100-300μL of the sample

6.  Acceptable Sample Types: serum, plasma, cultured cells, saliva, alveolar lavage fluid, nasopharyngeal aspirate, swab samples, etc.


 Performance Verification
1.  Validation of Nucleic Acid Extraction from Different Types of Samples

Sample No.

Sample Type

Sample Size

A260/280

A260/230

Nucleic Acid Vield (μg)

1

Nasopharyngeal aspirate

200 µl

1.84

1.95

4.2

2

Human serum

200 µl

1.84

2.03

1.8

3

Nasal swab

1 swab

1.87

2.19

2.3

4

Oral swab

1 swab

1.85

1.84

1.9

5

Saliva

200 µl

1.84

2.04

9.2

Note: the data in the table are obtained from testing the samples collected by our company’s labs, which cannot represent the yield of all samples of the sample type and are only for reference.

DNA/RNA Electropherogram of Different Sample Types

2.   Validation of Repeatability

Same batch of products. For the yield and purification data of DNA/RNA extraction from the same sample,  A260/280 within 1.8-1.9, A260/230＞2.0.


3.   Validation of Inter-lot Difference

Different batch of products. For the yield and purification data of DNA/RNA extraction from the same sample,  A260/280 within 1.8-1.9, A260/230＞2.0

Consumables can be purchased separately

1.  USP VI Polypropylene material;
2.  High chemical stability;
3.  Non-pryogenic, DNase/RNase free.

Cat. No.

Product Description

/Pack

/Cs

503621

NEST MagPureTM 
2.2 mL 96-Well Deep Well Plate, 
V-Bottom, Square Well, Compatible with Kingfisher Flex & Presto, Sterile

5

50

500621

NEST MagPureTM 
0.5 mL 96-Well Deep Well Plate, 
V-Bottom, Square Well, Compatible with Kingfisher Flex & Presto, Sterile

5

50

503361

NEST MagPureTM 96 Tip Combs for Magnetic Applications,
Compatible with Kingfisher Flex &Presto, Sterile

5

50


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