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Nucleic Acid Extraction Kit

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 Product Application
The kit is suitable for TECHSTAR YC769 Nucleic Acid AutomaticExtraction System for extracting genomic DNA/RNA of pathogenicmicroorganisms from samples such as serum, plasma, cultured cells,saliva, alveolar lavage fluid, nasopharyngeal aspirates and swabs.


 List of Product Composition

Product name

Amount

Capacity

Components of Pre-filled Solution

Tip comb


1 pcs

/

/

Sample Plate

1 pcs

500μL×96 wells

Guanidine Hydrochloride, TritonX-100, EDTA, etc


Beads plate


1 pcs

200μL×96 wells

Magnetic beads


Wash 1 plate


1 pcs

600μL×96 wells

Guanidine Hydrochloride, EDTA, etc


Wash 2 plate


2 pcs

600μL×96 wells

75% ethanol

Eluting Plate

1 pcs

100μL×96 wells

TE buffer

Protein K

1 mL x 2 pcs

/

Protein K (PK)


 Product Advantages

1.  Reliable stability：Unique "Protease K" stable at room temperature, the complete kit does not need to be stored at low temperature.
2.  High binding：Magnetic beads optimized for pathogenic microbial genomes have excellent adsorption effects on small nucleic acids.
3.  Strong sealin：High-viscosity sealing film prevents liquid leakage during transportation.
4.  High sensitivity：With strong reproducibility and high purity, it can recover trace amounts of pathogenic microorganism DNA/RNA.

 List of Product Composition


 Performance Parameter

1.   Extraction Purity: A260/280= 1.8-1.9, A260/230 >2.0

2.   Yield of Nucleic Acid: >85%

3.   Extraction Time: around 45 min

4.   Extracted Number: 96 samples for each run

5.   Sample Adding Amount: 100-300μL of the sample

6.  Acceptable Sample Types: serum, plasma, cultured cells, saliva, alveolar lavage fluid, nasopharyngeal aspirate, swab samples, etc.


 Performance Verification
1.  Validation of Nucleic Acid Extraction from Different Types of Samples

Sample No.

Sample Type

Sample Size

A260/280

A260/230

Nucleic Acid Vield (μg)

1

Nasopharyngeal aspirate

200 µl

1.84

1.95

4.2

2

Human serum

200 µl

1.84

2.03

1.8

3

Nasal swab

1 swab

1.87

2.19

2.3

4

Oral swab

1 swab

1.85

1.84

1.9

5

Saliva

200 µl

1.84

2.04

9.2

Note: the data in the table are obtained from testing the samples collected by our company’s labs, which cannot represent the yield of all samples of the sample type and are only for reference.

DNA/RNA Electropherogram of Different Sample Types

2.   Validation of Repeatability

Same batch of products. For the yield and purification data of DNA/RNA extraction from the same sample,  A260/280 within 1.8-1.9, A260/230＞2.0.


3.   Validation of Inter-lot Difference

Different batch of products. For the yield and purification data of DNA/RNA extraction from the same sample,  A260/280 within 1.8-1.9, A260/230＞2.0.


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